|
KMID : 1377020180150050639
|
|
Tissue Engineering and Regenerative Medicine
2018 Volume.15 No. 5 p.639 ~ p.647
|
|
|
Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics
|
|
Yu Na-Hee
Chun So-Young
Ha Yun-Sok
Kim Hyun-Tae
Kim Dae-Hwan
Kim Jeong-Shik
Chung Jae-Wook
Lee Jun-Nyung
Song Phil-Hyun
Yoo Eun-Sang
Kim Bum-Soo
Kwon Tae-Gyun
|
|
|
Abstract
|
|
|
BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions.
METHODS: To establish optimal conditions, human amniotic fluid stem cells’ (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1?×?107 cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells.
RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells.
CONCLUSION: Our findings can provide a foundation to optimization of conditions for stem cell transport.
|
|
|
KEYWORD
|
|
|
Cell transporting, Medium, Cell density, Amniotic fluid stem cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
   
|
|